Blue pippin

The Iso-Seq library was constructed using the Clontech SMARTer PCR cDNA Synthesis Kit and the Blue Pippin Size Selection System protocol, according to the Iso form Sequencing protocol (Iso-Seq), as described by Pacific Biosciences (PN100-092-800-03). Oligo magnetic adsorption was used to enrich Poly (A) mRNA. cDNA fragments > 4 bp in size were ...

Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Upgrading the Pippin Instrument Software. 71. 21 Preventative Maintenance - Rinsing Electrodes. 73. Electrode Rinse Procedure. 73. 22 Warranty and Service. 74. ... 3 Blue-Pippin Installation Procedure. 5. Packaging and Components. 5. Instrument and Monitor Dimensions. 5. Installation. 6. Rear Panel of the Bluepippin. 7.Hi, I am a kind of enthusiast and would like to improve data on my genome with nanopore long reads. Do you possibly know of any lab that performs…Pippin apples vary in availability, depending on the variety. Most cultivars are harvested in the late fall through early winter and can be stored until the spring. A few Pippin varieties may ripen in mid-summer. Current Facts Pippin apples, botanically classified as Malus domestica, are a category of apple varieties belonging to the Rosaceae ...The synthesized cDNA was enriched by PCR amplification, the amplified cDNA fragments were divided into different size fractions using the Blue Pippin size selection system (Pacific Biosciences).News and Events 17-04 Reportage NRC handelsblad: 'Is het een schimmel, is het een virus?Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center. 22-12 PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and …Monarch HMW DNA Extraction Kit produces high molecular weight DNA with excellent yields, purity, and fragment length as compared to other commercially available kits. HMW was isolated from 1x106 HEK293 cells and fresh human blood with the kits indicated in the figure legend. Blood input volumes were used as specified in manufacturers ...

Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was used for the size selection of different sizes of cDNA libraries (1–2, 2–3, and > 3 kb). According to the instructions, three SMRT libraries were constructed using SMRTbell template prep kit 1.0 (Pacific Biosciences) and were sequenced through seven SMRT …Molecular Biology. Schedule an Instrument. CORE Director: Rob Miller, PhD Manager: Melissa Sanchez, PhD Bioinformatics Specialist: Lijing Bu, PhD. Phone: (505) 277-4278. The Molecular Biology Core located within the Department of Biology at the University of New Mexico, provides genetic sequencing services to University investigators and maintains a repository of equipment for use by faculty ...The synthesized cDNA was enriched by PCR amplification, the amplified cDNA fragments were divided into different size fractions using the Blue Pippin size selection system (Pacific Biosciences).The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.The Iso-Seq library was constructed using the Clontech SMARTer PCR cDNA Synthesis Kit and the Blue Pippin Size Selection System protocol, according to the Iso form Sequencing protocol (Iso-Seq), as described by Pacific Biosciences (PN100-092-800-03). Oligo magnetic adsorption was used to enrich Poly (A) mRNA. cDNA fragments …Flexible, movable furniture often feels temporary, missing the comfort factor – not so with Pippin. This single-seat, cone-shaped lounge chair offers a.

from BioRad and Pippin Pulse System from Sage Science). Figure 2. Low DNA Input Sample and SMRTbell Library Preparation Workflow. To sequence a single low DNA input sample, ≥400 ng of input gDNA is required for the Sequel II System and the target DNA shear size distribution is 12 kb - 20 kb using the Megaruptor system. The library preparationThis blue hat is appropriate to wear for the cool weather races, church, dress occasions, weddings and more. 1 in stock. Pippin quantity. Add to cart. SKU: ...polymyxa DSM36 native genomic DNA (2 μg) was obtained directly from the DSMZ collection and a 15kb SMRT bell library was prepared according to the Pacific Biosciences 10-20 kb protocol including additional separation using a Blue Pippin and sequenced with C4-P6 chemistry on 2 SMRT cells. One library consisted of non-size selected (15 kb) DNA ...Pippin depicted everyday events, historical figures, and religious themes using a simplified style of flat shapes and strong colors. In the American popular press of the 1910s and 1920s, the blue tiger was a recurring symbol of the unattainable, and the black bear signified the wilderness. The tiger and bear, rendered in contrasting tones, seem ...To anchor the scaffolds to chromosomes, 26.79 Gb of clean reads (65.50×) were obtained via Hi-C library sequencing (Supplemental Table 5).Read pairs were mapped to the draft assembly using BWA (version .7.10-r789) (Li and Durbin, 2009).We determined that 85.28% of the reads were correctly mapped to the genome (Supplemental Table 6), including 22.01% uniquely mapped read pairs.

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Are you looking to purchase a used RV? If so, you’ve come to the right place. Kelley Blue Book is the go-to source for used vehicle pricing and research, and they have a wealth of information to help you get the most out of your used RV pur...For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...Page 2 P/N 100-286-000-07 STEP Concentrate DNA Notes 1 Add 0.45X volume of AMPure® PB magnetic beads. _____ μL of sample X 0.45X = _____ μL of beads Note that the beads must be brought to room temperature and all AMPure PB beadSee full list on sagescience.com Internal price (Cornell and Cornell affiliates) External price. Fragment analysis, ready-to-load, per 96-well plate. $42.50. $69.70. LIZ500 and HiDi Formamide addition, per 96-well plate. $40.00. $65.60. ROX500 and HiDi Formamide addition, per 96-well plate.

The Blue Pippin system . from Sage Science enables targeted size selection. High-pass filtering kits for the Blue Pippin are beneficial for long-read sequencing, as they allow the user to collect all the sample above a specified threshold, enabling analysis of only the fragment length of interest. Size selection therefore makes it possible to4. Follow the manufacturer’s recommendations to set up a run protocol. a. Select the “0.75%DF Marker S1 High-Pass 6–10kb vs3” Cassette Definition File. b. Using the “Range” selection mode, enter a desired “BPstart” value of 10000 and a “BPend” value of 50000.For a subset of samples, 3 µl of each was pooled and concentrated for 450-650 bp size selection using a Blue Pippin 1.5% agarose cassette with R2 marker. The average size of the recovered ...Blue Pippin Standard DNA size selection cassette 2% agarose The blue pippin is a machine that is used to size-select DNA fragments from 100bp-50kbp.Target sizes or rangesof sizes are entered in software, and fractions are collected in buffer.Buckley lab protocols for conducting SIP studies. Contribute to nick-youngblut/Buckley_Lab_SIP_project_protocols development by creating an account on GitHub.The genomic DNAs were broken up by 26G Needle, and subsequently, the fragments above 10 kb were selected by Blue Pippin. After terminal repair and A-tailing, the ends of the fragments larger than 10 kb were ligated to generate the DNA library. The library sequencing was performed on a Pacbio sequel platform (Pacific Bioscience, California, …The 20 kb templates used for sequencing were prepared by using Blue Pippin Size-Selection System (Sage Science, Beverly, MA) that allows resolving and collecting high molecular weight DNA, according to the manufacturer’s instructions. DNA Sequencing and Assembly. The genomic sequence of Bt 2 Annotation of coding …Blue/Green LEDテクノロジー; Blue/Green LED 搭載ゲル撮影装置; FAS-Digi PRO はゲル撮影の新時代を切り開く！ ibidiアプリケーションガイド集; クリニカルシーケンス技術特集; 情報をさがす; メディアサイト UP! Online（外部） 新製品・サービス; キャンペーン・モニター ...the gel. The analytical gel was run with a Pippin Pulse using the 10-48kb pre-set protocol and run for 15 hours. 0.75% SeqKem Gold agarose from Lonza was used. 4 kb 5 kb 8 kb 6 kb 10 kb None (Control DNA) 6 kb 7 kb 8 kb 10 kb High-Pass threshold setting 29 kb 36 kb Marker S1 At the end of a run, marker peaks will be detected in designated ...The desired product was confirmed by running agarose gel (0.8%) electrophoresis and purified by using Blue pippin (Sage Science) (Supplementary Fig. 1b). Single-molecule distance measurement using ...2. Scientists and Bioinformaticians. • Interested in Experimental Design and De Novo assembly using. the PacBio® HGAP method. After the training, you will be able to. • Understand how the HGAP method works. • Understand the coverage targets for de novo assembly with PacBio. data. • Import and parameterize an HGAP assembly job in SMRT ...

Fragment size may be measured using several methods (e.g., pulse-field, low percentage agarose gel analysis, blue pippin). This quality checkpoint . is important during the development and validation of the test. Labs may elect to omit this quality check after validation if the test has proven robust and stable.

Individuals were then pooled, followed by a size selection step with the Blue Pippin (Sage Science, Beverly, MA) where all loci between 415 and 515 bp were retained. A final PCR step using Phusion Taq polymerase (New England Biolabs) with the following thermocycler conditions was conducted to amplify all loci and attach a 6bp index unique to ...The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing platforms. Features : Saves time and effort compared to manual gel purification; Sample-to-sample reproducibility streamlines researchBlue Pippin size selection 250bp - 1.5kb Size select samples using the Blue Pippin 1.5% agarose cassette, between 250 bp and 1.5 kb. Smaller size ranges will have lower yields. Cost is per sample. A normal cassette cartridge contains 5 sample lanes with internal standards. Internal $98.00 each External $117.60 each Corporate $162.97 each[PIPPIN] My days are brighter than morning air Evergreen pine and autumn blue But all my days are twice as fair If I could share My days with you My nights are warmer than firecoals Incense and stars and smoke bamboo But nights were warm beyond compare If I could share My nights with you To dance in my dreams To shine when I need the sun With youThe BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ... Find the nearest stores. Search Address. Use current location. Seiko Prospex remains a standard for high-intensity timekeeping excellence. From the introduction of its first diver’s watch in 1965, Seiko has been a leader in undersea precision and durability, spearheading innovations in both technology and design.Are you looking to get the most out of your 2007 Honda Civic? The Blue Book is a great resource to help you do just that. The Blue Book is an online resource that provides information on the value of used cars, including the 2007 Honda Civi...DNA Size Selection System Operations Manual Software v.6.22 Cassette Definition Set 19

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A man in a red coat in a spotlight in front of a blue background with. Pippin. Nebraska Repertory Theatre. April 1-11, 2020. Watch Video. CLOSE X. light. This ...Sage Science, Pippin Prep, Blue Pippin, and Fill & Sign Online, Print, Email, Fax, or Download Get Form Form Popularity . Get Form eSign Fax Email Add Annotation Share Not the form you were looking for? Search. Сomplete the Suite 3150 for free Get started! Rate free . 4.4. Satisfied. 21 ...It's important to carefully inspect gel cassettes prior to use on the Pippin Prep or BluePippin. This instructional video from Sage Science shows how to quic...This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...As a new type of fish, GF-L had a high fertilization rate (91.62%) and a hatching rate (82.67%). GF-L presented very beautiful phenotypes, such as twin tails, blue eyes and a white body accompanied by red spots. These phenotypes were very different from any other GF phenotype, showed that the GF-L lineage had great potential in the ornamental ...The core facility staff will wear face mask in the laboratory, which hosts multiple instruments for shared use by researchers via scheduling. Users are required to follow the University's COVID-19 Policies and Guidance on this web page: The primary contact for the Genomics Core Facility is Wei Wang, the Director, [email protected], 609-258-6998.Conductivity / pH Meter. GE Biacore 3000 Surface Plasmon Resonance (SPR) Malvern NanoSight NS300. Malvern Zetasizer Nano ZS. Milli-Q Advantage A10 Water System. MiSonix Ultra Sonicator. New Brunswick Innova 44R Shakers. Other Analytical Core Instruments. Shimadzu UV-1800 UV-Vis Spectrophotometer.Safety Data Sheet In accordance with Regulation (EC) No 1272/2008 (REACH) Trade name: BluePippin™ Agarose Gel Cassettes Product No: BDQ3010, BEF2010, BDF2010, BDF1510, PAC20KB, PAC30KB,8. Nike Air Flight '89. The Nike Air Flight '89 had a design that was slightly based on the Air Jordan 4 and is one of Pippen's sneakers that has endured the test of time, still available on ... ….

The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using “tight” settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...Molecular characterization is a key step in the risk assessment of genetically modified organisms (GMOs) for regulatory approval. Herein, we describe a method for analyzing copy number, insertion loci, and flanking sequences through whole-genome sequencing (WGS) and bioinformatics. Comprehensive molecular characterization of G2-6 transgenic rice was performed using this pipeline. The results ...Blue Pippin Covaris M220 UV-Vis | Tecan Plate Reader Malvern NanoSight | Zetasizer Qubit 2.0 Analytical Core Other Services Reads 600 bp (8 Samples) 5 Microscope Nano NextSeq 500 75 bp 150 bp (PE75) 300 bp (PE150) Output Level SequencingHigh Mid 400 M 130 M Yield 30 Gb 19.5 Gb 60 Gb 39 Gb UCSB Rate NextSeq 500 -details on reverse sideSelection Chart Pippin Prep BluePippin PippinHT Max. DNA input 5 μg 5 μg 1.5 μg Max. Sample Capacity 5 5 24 Pulsed-Field No Yes Yes Run Times (100 bp - 1.5 kb) 50-90 min 50-90 min* 25-45 min* Size Selection Range 100 bp - 1.5 kb 100 bp - 50 kb 100 bp - 1.5 kb High-Pass Size Filtering No Yes Yes Low-Pass Size Filtering Yes Accuracy > 93% ... 3.0 BLUE-PIPPIN INSTALLATION PROCEDURE 3.1 Packaging and Components The system is shipped in two boxes: one will contain the BluePippin instrument and accessories, and the second box will contain the computer monitor in the manufacturer's original packaging. With the boxes in the upright position, open and confirm that the following items are ...For PacBio libraries, we followed the study of Chakraborty et al. by shearing the DNA using a 1.5-inch 24-gauge blunt-tipped needle, and the library was prepared using PacBio's SMRTbell template protocol and Blue Pippin size selection using a 15- to 50-kb cutoff. We generated several dozen libraries in this manner and collected 124 total ...Size-selection was performed using the Blue Pippin electrophoresis platform (Sage Science), in order to select fragments of 215 bp of length as in [ 70 ], in order to be able to integrate our data ...Background Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests. In contrast, genomic information for crop pests is ...As a new type of fish, GF-L had a high fertilization rate (91.62%) and a hatching rate (82.67%). GF-L presented very beautiful phenotypes, such as twin tails, blue eyes and a white body accompanied by red spots. These phenotypes were very different from any other GF phenotype, showed that the GF-L lineage had great potential in the ornamental ...Pipis first appear as oval-shaped blue objects, resembling a balloon or an egg. When they pop, dozens of tiny Spamton faces fly out in every direction. Starting from version v1.07 there is a 1/100 chance of a "Ms. Pipis" replacing a regular Pipis, which sports a simple pink bow, but is otherwise identical to Pipis. Personality [] Blue pippin, [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1]