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Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that …

Promega us. Add to Cart. P450-Glo™ CYP1A1 Assay System. 10ml. $ 223.00. Your price: Log in. The P450-Glo™ CYP1A1 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP1A1 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes.

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important.

The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay ...An Introduction to Cell Health Assays. Cell health assays are used to detect and monitor various cellular events and build a picture of how and why cells live or die. They are used for many different applications including assessing toxicity of drug candidates and other compounds, analyzing cellular changes in cancer and other diseases, and ...Promega was named a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. This award recognizes Promega for not only offering great benefits, but for putting employees first, unlocking their potential and inspiring their growth and performance. Over the past year, Promega has been recognized with additional ...Promega’s Training Support Program offers a generous 50% price discount to instructors using DNA, RNA, protein or cell-based techniques in their course. Discover the Benefits. With the Helix on-site stocking program, you'll have easy, 24/7 access to the reagents and products you need. No hassle, no paperwork, just more time to focus on science.The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). Simple, homogeneous protocol: After addition of 2DG, there are no wash steps—all steps are additions. Sensitive with broad linearity: The Glucose Uptake …

Sensitive, Rapid and Convenient Dual-Reporter Assay. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly ( Photinus pyralis) and Renilla ( Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The Maxwell ® RSC miRNA Tissue Kit purifies total RNA, including microRNA (miRNA), from 1–48 tissue samples in about 90 minutes. The Maxwell ® RSC miRNA Plasma and Serum Kit will purify total RNA, including miRNA, from 1–48 samples in about 70 minutes. The kits provide all the reagents required for sample processing and use prefilled ... The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important.Madison, WI USA. (March 27, 2023) Promega has been named a top employer in the region, winning a 2023 Madison Area Top Workplaces award this week from The Wisconsin State Journal. The biotechnology manufacturer ranked second in the category of large employers in the region and was honored at the national level earlier this year with a … The CellTiter-Glo® 2.0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening. High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in ... Join us at Drug Discovery Chemistry 2024 to see the latest solutions in cellular target engagement for DDR pathway targets including PRMT5, Polymerase Theta, PARG and PARP. ... Promega Corporation ...Promega Corporation provides biological and pharmacy products. The Company offers reagents, instrumentations, and enzyme systems. Promega serves customers worldwide. Company profile page for ...

The Medical Science Liaison is a professional who serves as a key field-based scientific resource for these clients and internal Promega colleagues. CORE DUTIES: 1. Engage in non-promotional peer-to-peer exchange of medical and scientific information with Scientific Leaders or Healthcare Professionals. 2. About Promega. We are a leader in providing innovative solutions and technical support to the life sciences industry. Promega Corporation’s 2,000 products enable scientists …Angus. DNA and RNA extraction, plasmid purification, reporter assays, pcr, qpcr, cloning enzymes, cell viability assays, apoptosis detection systems, luminometers, protein expression systems and more. Links to the entire contents of the Promega product catalog are accessible from this page.Comparison of DNA yields using the Wizard® SV and SV 96 Genomic DNA Purification Systems. Average yield of genomic DNA in micrograms purified from 20mg mouse tail clippings. The average A 260 /A 280 ratios are: SV … Working at Promega means growing and developing with a very innovative company that stands for progress and knows how to combine economic and social aspects. The cultural diversity and scientific inspiration make the company a place where people enjoy working. Caroline End, Product Manager, Promega Germany. Promega Corporation careers and job ... Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. DNA extraction kits are available for all sample throughput needs–from simple manual systems through benchtop automation to customizable chemistries for DNA isolation on ...

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A1120, A1125, A1620. Literature # TM050. The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, Gram-positive and Gram-negative bacteria. The Wizard® Genomic DNA Purification Kit is based on a four-step process. Contact Us. Peek inside the mind of the scientist who created our amazing scientific paintings. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological ...Protocols for Promega products. Includes Technical Manuals, Technical Bulletins, Product Information Sheets, Protocol Cards and Automated Protocols for high-throughput systems. ... We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies ... A1120, A1125, A1620. Literature # TM050. The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, Gram-positive and Gram-negative bacteria. The Wizard® Genomic DNA Purification Kit is based on a four-step process. Add to Cart. P450-Glo™ CYP1A1 Assay System. 10ml. $ 223.00. Your price: Log in. The P450-Glo™ CYP1A1 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP1A1 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes.

GloMax® Navigator is an easy-to-use microplate luminometer integrated with Promega chemistries for superior luminescence assay performance. The instrument comes ready to use—simply unpack it, plug it in and begin your experiments. You can also interpret results using integrated data analysis software. GloMax® Navigator provides superior ... Contact Us. Peek inside the mind of the scientist who created our amazing scientific paintings. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological ... Protein purification is a fundamental step for analyzing individual proteins and protein complexes. Escherichia coli remains the first choice of many researchers for producing recombinant proteins due to ease of use, rapid cell growth and low cost of culture. Proteins expressed in E. coli can be purified in relatively high quantities, but these ...A1222, A1223. Literature # TB374. The PureYield™ Plasmid Miniprep System isolates high-quality plasmid DNA for use in eukaryotic transfection and in vitro expression experiments. The system provides a rapid method to purify plasmid DNA using a silica membrane column. Plasmid DNA can be purified in less than 10 minutes, depending on …Promega North America Promega North America 2800 Woods Hollow Road Madison, WI 53711 Contact Us United States Marketing Area: Canada. Tel: 608-274-4330 Fax: 608-277-2516 Toll-Free Phone: 800-356-9526 Toll-Free Fax: 800-356-1970 E-mail Address: [email protected] E-mail ... Technician 1, IVD Product Finishing (1st Shift) Req ID: 3684. Location Promega Madison Madison, Wisconsin. Categories Manufacturing & Production. Position Type Regular Full-Time. Apply Now. About Promega. We are a leader in providing innovative solutions and technical support to the life sciences industry. Promega Corporation’s 2,000 products enable scientists …The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.#. V3021).The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format. The 11 amino acid HiBiT peptide tag can be added easily to a protein of interest, and the total amount of HiBiT-tagged protein in cells measured by adding the Nano-Glo ...STR amplification systems can accommodate a range of template DNA concentrations. Most of the Promega PowerPlex® STR systems provide optimal sister allele balance and locus-to-locus balance with 0.5–1.0ng of DNA template. However, amplification and detection instrumentation can vary. You may need to optimize protocols, including cycle number ...Feb 15, 2024 · Madison, WI USA. (February 15, 2024) The Promega Spring Art Showcase will explore the internal landscapes of the mind in an exhibition titled “Visions of Consciousness.”. The exhibit will portray how consciousness shapes our perception of reality through representations of thoughts, dreams and emotions. “Visions of Consciousness” opens ...

Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. DNA extraction kits are available for all sample throughput needs–from simple manual systems through benchtop automation to customizable chemistries for DNA isolation on robotic …

The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay ...Add to Cart. P450-Glo™ CYP1A1 Assay System. 10ml. $ 223.00. Your price: Log in. The P450-Glo™ CYP1A1 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP1A1 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes.The results of compound screening using the CellTiter-Glo® 3D Assay in hanging-drop, ultra-low attachment plate (ULA) and Matrigel® 3D cultures are shown below. Equivalent results were achieved for all three methods. HCT116 colon cancer cells were seeded as follows: 400 cells in hanging-drop; 1,000 cells in ULA or Matrigel®.High Sensitivity. The increased light output of the Steady-Glo® Assay provides improved sensitivity over other extended-lifetime firefly luciferase assay reagents. Convenient and Easy to Use. To prepare the reagent, simply mix buffer with lyophilized substrate. Add the reagent directly to cells in culture, wait 5 minutes and measure luminescence.Here, you’ll find a range of nucleic acid extraction kits that generate high yields from many sample types. From single-prep manual solutions, to cartridge-based Maxwell® Systems, to plate-based processing compatible with high throughput liquid handling systems—Promega chemistries cover the range of your purification needs.Sustainable Working Environment for Career Starters and Experienced Professionals. Founded in 1978 in Madison, Wisconsin, USA, Promega now has branches in 16 countries and more than 50 global distributors. Since its foundation, Promega has become one of the world's biggest manufacturers of reagents, kits and device systems in the life science ...RT-PCR kits and standalone reverse transcriptase enzymes for reverse transcription of full-length cDNA from your experimental sample. Includes GoScript™ Reverse Transcriptase, M-MLV and AMV reverse transcriptases. GoScript uses M-MLV reverse transcriptase and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and …

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1 each. $ 182.00. Your price: Log in. The Vac-Man® Laboratory Vacuum Manifold is a sturdy, chemical-resistant manifold capable of processing 20 samples simultaneously. Each Vac-Man® Manifold is supplied with 20 individual Luer-Lok® Stopcocks, so each port is controlled individually. The Spinlock II Adapters can be attached to the upper row ...Webinars cover educational topics in life science research, drug discovery, clinical research, applied sciences, clinical and molecular diagnostics, and more. Live presentations include the opportunity to ask questions via chat. Don't worry if you missed a presentation date—past Webinars are available for on-demand viewing. Working at Promega means growing and developing with a very innovative company that stands for progress and knows how to combine economic and social aspects. The cultural diversity and scientific inspiration make the company a place where people enjoy working. Caroline End, Product Manager, Promega Germany. Promega Corporation careers and job ... UK. Purification kits for extraction of viral RNA and viral DNA. This kit extracts total nucleic acid (DNA and RNA) from a variety of viral sample types. This is a cartridge-based kit that is used with the Maxwell® Instruments to automate isolation of viral nucleic acids from up to 48 samples per run.Promega was named today as a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. Companies earn a place on the Top Workplaces USA list based solely on feedback from employee engagement surveys compiled by research firm Energage. More than 1,200 companies across four size …100ml. Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles. Glo Lysis Buffer is fully compatible with Bright-Glo™, Steady-Glo®, ONE-Glo™ and Renilla -Glo® Luciferase Assay Reagents and the Luciferase Assay ...Reporter Assays. A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer.Some intracellular luciferases, such as NanoLuc® and Renilla luciferases, can be detected without cell lysis to create live-cell reporter assays that measure the kinetics of a cellular response. Most reporter gene assays involve either one reporter gene or two. Most often, the second reporter is expressed from a "control" vector to normalize ... ….

Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. Our product portfolio of over 4,000 products supports a range of life science work in cell biology, DNA, RNA and protein analysis, drug development, human identification and molecular diagnostics. Promega products and technologies are used …Promega Corporation. @promega ‧ 5.55K subscribers ‧ 174 videos. At Promega Corporation, creativity and connection drive discovery and innovation....100ml. Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles. Glo Lysis Buffer is fully compatible with Bright-Glo™, Steady-Glo®, ONE-Glo™ and Renilla -Glo® Luciferase Assay Reagents and the Luciferase Assay ... HiBiT technology simplifies protein tagging in live cells, providing a streamlined protocol for detecting tagged proteins that requires only a luminometer for detection. With the dynamic range to detect proteins in live cells without overexpression, and the convenience of a single-reagent-addition method, HiBiT technology opens up a universe of ... News and Press Releases. Promega Corporation is a global biotechnology company headquartered in Madison, WI, with branches in 16 countries. Our products and technologies are used to support scientific advances in academic, pharmaceutical, clinical, forensic and industrial/environmental labs worldwide. The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format. The 11 amino acid HiBiT peptide tag can be added easily to a protein of interest, and the total amount of HiBiT-tagged protein in cells measured by adding the Nano-Glo ...Promega US Sales/OEM 2800 Woods Hollow Road Madison, WI 53711 Media Inquiries Penny Patterson (608) 277-2642 [email protected] Contact Us United States Marketing Area: United States, Canada. Tel: (608) 274-4330 Fax: (608) 277-2516 Toll-Free Phone: (800) 356-9526GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.Promega nucleic acid extraction chemistries cover a full range of sample throughput needs--from single-prep manual solutions through benchtop automation to plate-based systems compatible with robotic liquid handlers. ... We use these cookies to collect information about how you interact with our services and to help us measure and … Promega genomic DNA extraction products are designed for isolation of high-quality DNA from many sample types, including blood, cells, tissues, plants, microbes and more. DNA extraction kits are available for all sample throughput needs–from simple manual systems through benchtop automation to customizable chemistries for DNA isolation on ... Promega us, [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1], [text-1-1]